| 发明名称 |
DNA plasmids with improved copy number |
| 摘要 |
The present invention relates to the production of covalently closed circular (ccc) recombinant DNA molecules such as plasmids, cosmids, bacterial artificial chromosomes (BACs), bacteriophages, viral vectors and hybrids thereof, and more particularly to vector modifications that improve production yield of said DNA molecules in fermentation culture. |
| 申请公布号 |
US9045759(B2) |
申请公布日期 |
2015.06.02 |
| 申请号 |
US201012656115 |
申请日期 |
2010.01.19 |
| 申请人 |
|
发明人 |
Williams James Arthur |
| 分类号 |
C12N15/00;C12N15/69;C12N15/70;C12N1/20 |
主分类号 |
C12N15/00 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A PAS-BH region pUC origin plasmid vector composition, comprising:
a PAS-BH region comprising at least 90% sequence identity to the sequence set forth as SEQ ID NO: 4, and a pUC origin plasmid vector wherein said PAS-BH region is operably linked to said pUC origin and wherein said PAS-BH region pUC origin plasmid vector does not comprise the repressor of primer (ROP) gene and wherein plasmid copy number of said PAS-BH region pUC origin plasmid vector is increased in shake flask or fermentation culture of transformed bacterial cells compared to said pUC origin plasmid vector. |
| 地址 |
|
