Generating a mucin-producing cell from an umbilical cord amniotic membrane epithelial stem cell

摘要

The present invention relates to the generation of a mucin-producing cell using stem/progenitor cells obtained from the amniotic membrane of umbilical cord and therapeutic uses of such mucin-producing cells.

主权项

1. A method of generating a mucin-producing cell comprising:
placing umbilical cord amniotic membrane epithelial stem cells (UCEC) in a container; culturing the UCEC in the container in a growth medium adapted for the cultivation of a mucin-producing cell through the addition of a growth factor selected from the group consisting of epidermal growth factor (EGF), insulin-like growth factor-1, platelet-derived growth factor-BB (PDGFb), transforming growth factor-β, keratinocyte growth factor (KGF), TGF-αand amphiregulin and combinations thereof; and permitting the UCEC to differentiate into mucin producing cells as indicated by observing mucin in the culture medium.