| 摘要 |
FIELD: medicine.SUBSTANCE: invention relates to immunology and medical diagnostics and represents a method of the immunochromatographic determination of specific antibodies. The method is based on the contact of a membrane test-strip with an analysed liquid sample and initiated by the said contact movement of reagents, contained in the sample or applied on the membrane and forming immune complexes in the course of interactions in the membrane pores or on its surface, on the test-strip membranes. The intensity of colouration of a mark, which bound in the analytical zone, is detected visually. Characteristic peculiarity of the claimed method of the antibody determination consists in the fact that a conjugate of colloid gold with an antigen is used in the test, and the reagent for binding common antibodies is immobilised in the analytical zone of the test-strip, whereas in a standard scheme of immunochromatography for the determination of specific antibodies the reagent for binding antibodies is conjugated with colloid gold and the antigen is immobilised in the analytical zone of the test-strip.EFFECT: application of the claimed method for analysis makes it possible to increase the analysis sensitivity and/or reduce the antigen consumption.2 dwg, 1 ex |
