分析样本中内毒素之方法;METHOD FOR DETECTING ENDOTOXIN IN A SAMPLE

摘要

一种分析样本中内毒素之方法，包括提供样本，此样本包括内毒素(endotoxin)；加入几丁聚醣(chitosan)磁性奈米粒子于样本中，几丁聚醣磁性奈米粒子专一性结合至内毒素并形成错合物；将外部磁铁靠近样本之第一部分，使样本中的错合物聚集于第一部分；移除样本之第二部分，此第二部分与第一部分不同；对第一部分中的内毒素进行质谱分析步骤。; adding chitosan magnetic nanoparticles into the sample, wherein the chitosan magnetic nanoparticles specifically bind the endotoxin to form a complex; approaching an external magnetic to a first portion of the sample to concentrate the complex to the first portion; removing a second portion of the sample other than the first portion; and analyzing the endotoxin in the first portion by mass spectrometry.