发明名称 |
PDMS Membrane-Confined Nucleic Acid and Antibody/Antigen-Functionalized Microlength Tube Capture Elements, and Systems Employing Them |
摘要 |
A microfluidic device comprising a microfluidic channel network sealed on one side by a membrane sheet, the sheet having PDMS defining at least the surface sealing the channel, the membrane sheet on its opposite side sealing one side of a pneumatic channel, the pneumatic channel arranged to enable pneumatic deflection of a deflectable portion of the membrane sheet into contact with an opposed surface to control flow in a channel of the network, the membrane sheet confining in a channel of the network at least one micro-particle, micro-length tube or glass nano reactor, functionalized with a capture agent, that has been inserted into that channel. A microfluidic device having a microfluidic channel containing at least two micro-particles, micro-length tubes or glass nano reactors, one functionalized with nucleic acid and another with antibody or antigen. A microfluidic device having a microfluidic channel containing at least one micro-length tube or glass nano reactor functionalized to capture nucleic acid, the device constructed to enable recovery of the nucleic acid captured by the device. |
申请公布号 |
US2015087559(A1) |
申请公布日期 |
2015.03.26 |
申请号 |
US201414479288 |
申请日期 |
2014.09.06 |
申请人 |
CyVek, Inc. |
发明人 |
Putnam Martin A.;Leamon John H.;Branciforte Jeffrey T.;Stanwood Charles O. |
分类号 |
G01N33/543;B01L3/00 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
1. A microfluidic device comprising a microfluidic channel network sealed on one side by a membrane sheet, the sheet having PDMS defining at least the surface sealing the channel, the membrane sheet on its opposite side sealing one side of a pneumatic channel, the pneumatic channel arranged to enable pneumatic deflection of a deflectable portion of the membrane sheet into contact with an opposed surface to control flow in a channel of the network, the membrane sheet confining in a channel of the network at least one micro-particle, functionalized with a capture agent, that has been inserted into that channel. |
地址 |
Wallingford CT US |