Process for producing reveromycin A or a synthetic intermediate thereof, process for producing compounds containing a spiroketal ring and novel antineoplastics, fungicides and therapeutic agents for bone disorders

摘要

A bacterium belonging to the genus Streptomyces having an ability to produce reveromycin A or a synthetic intermediate thereof, the bacterium being modified so as to increase expression of revQ gene coding for the amino acid sequence of SEQ ID NO: 36 or an amino acid sequence having an identity of not less than 80% to SEQ ID NO: 36 as compared with a parent strain, thereby the above-mentioned production ability is increased as compared with the parent strain.

主权项

1. A bacterium belonging to the genus Streptomyces having an ability to produce reveromycin A or a synthetic intermediate thereof,
wherein the synthetic intermediate is selected from the group consisting ofwherein the bacterium has been modified so as to increase expression of a revQ gene encoding (i) the amino acid sequence of SEQ ID NO: 36 or (ii) an amino acid sequence having not less than 90% identity to SEQ ID NO: 36 as compared with a parent strain, such that production ability of reveromycin A is increased as compared with the parent strain, and expression of the revQ gene has been increased by enhancing the copy number of the revQ gene or by modifying a promoter of the revQ gene.