ENGINEERED ZINC FINGER PROTEINS TARGETING 5-ENOLPYRUVYL SHIKIMATE-3-PHOSPHATE SYNTHASE GENES

摘要

The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.

主权项

1. A method for stimulating intramolecular homologous recombination in the genome of a plant cell, the method comprising the step of:
introducing a zinc finger nuclease (ZFN) into the plant cell in the presence of a DNA segment comprising a 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) gene and a first sequence that is homologous to a second sequence in the plant cell such that the ZFN cleaves the DNA segment and stimulates intramolecular homologous recombination, wherein the ZFN comprises a non-naturally occurring zinc finger protein (ZFP) that binds to a target site in the EPSPS gene, the zinc finger protein comprising the zinc finger domains set forth in any single row of Table A.