发明名称 |
RNA PREPARATIONS COMPRISING PURIFIED MODIFIED RNA FOR REPROGRAMMING CELLS |
摘要 |
The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-strand mRNA encoding an iPS cell induction factor. The purified RNA preparations are preferably substantially free of RNA contaminant molecules that: i) would activate an immune response in the somatic cells, ii) would decrease expression of the single-stranded mRNA in the somatic cells, and/or iii) active RNA sensors in the somatic cells. In certain embodiments, the purified RNA preparations are substantially free of partial mRNAs, double-stranded RNAs, un-capped RNA molecules, and/or single-stranded run-on mRNAs. |
申请公布号 |
SG10201408129X(A) |
申请公布日期 |
2015.02.27 |
申请号 |
SGX10201408129 |
申请日期 |
2010.12.07 |
申请人 |
THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA |
发明人 |
KARIKO, KATALIN;WEISSMAN, DREW;DAHL, GARY;PERSON, ANTHONY;MEIS, JUDITH;JENDRISAK, JEROME |
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