DETECTION METHOD FOR BIOLOGICAL SUBSTANCE

摘要

It is an object of the present invention to provide a detection method in which background noise is not increased even if a high-luminance fluorescent labeling material is used and which has an improved S/N ratio and high quantitativity and is advantageous for an immunohistological staining method. The present invention provides a detection method which is a method for detecting a specific biological substance using, as a color former, fluorescent substance-encapsulated nanoparticles to whose particle surfaces biological substance-recognizing molecules that specifically recognize a specific biological substance have been bonded, and which has an improved S/N ratio and high quantitativity and is advantageous for an immunohistological staining method because nanoparticles encapsulating no fluorescent substance are used as a blocking agent for preventing the fluorescent substance-encapsulated nanoparticles from being non-specifically adsorbed on a biological substance other than the specific biological substance.

主权项

1. A detection method for a specific biological substance, using, as a color former, fluorescent substance-encapsulated nanoparticles to whose particle surfaces biological substance-recognizing molecules that specifically recognize a specific biological substance have been bonded, wherein nanoparticles encapsulating no fluorescent substance are used as a blocking agent for preventing the fluorescent substance-encapsulated nanoparticles from being non-specifically adsorbed on a biological substance other than the specific biological substance.