| 摘要 |
FIELD: biotechnology.SUBSTANCE: ganglia of cephalopods are homogenised by double sonication. The obtained homogenate is diluted with distilled water, the mixture is filtered, centrifuged and the supernatant is passed through a chromatography column, where the sorbent is used as concanavalin A-sepharose (Con-A-sepharose), and dried, and prior to drying the propionylcholinesterase the solution of polyethylene glycol is added to the enzyme solution. The enzyme solution is sublimated at a temperature of final drying not exceeding 30°C.EFFECT: obtaining propionylcholinesterase with optimal specific activity on industrial scale.2 cl, 1 ex |
