Optical determination of glucose utilizing boronic acid adducts

摘要

The present invention concerns an improved optical method and optical sensing device for determining the levels of polyhydroxyl-substituted organic molecules in vitro and/or in vivo in aqueous media. The range of detection is between about 400 and 800 nm. In particular, a sensory devise is implemented in a mammal to determine sugar levels. Specifically, a dye is combined with a conjugated nitrogen-containing heterocyclic aromatic boronic acid-substituted bis-onium compound in the presence of a sugar, such as fructose or glucose. The viologens are preferred as the aromatic conjugated nitrogen-containing boronic acid substituted compounds. The method is useful to determine sugar levels in a human being.

主权项

1. An analyte sensor, comprising:
a fluorophore dye comprising a pyrene sulfonate
selected from the structure: wherein R1, R2, and R3 are each —NHR4, R4 is —CH2—CH2(—O—CH2—CH2)n—X1;wherein X1 is —OH, OCH3—CO2H, —CONH2, —SO3H, or —NH2; and n is between 70 and 10,000; and a quencher comprising a viologen substituted with at least one boronic acid; a semi-permeable membrane.