Surface neutralization of apatite

摘要

The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution.

主权项

1. A method for purifying a target molecule in a sample, the method comprising,
(a) equilibrating an apatite solid surface with a buffer composition suitable to adsorb the target molecule on the apatite solid surface and then contacting the sample comprising the target molecule to the apatite solid surface thereby adsorbing the target molecule to the solid surface; (b) after step (a), contacting the solid surface comprising the adsorbed target molecule with a solution having a pH of 7.5-9.0, the solution comprising:
(i) a basic amino compound and an alkali metal ion; or(ii) a sulphonated amine compound and an alkali metal ion, of sufficient volume and concentration to neutralize the apatite solid surface wherein the solution has a sufficiently low ionic strength such that the target molecule remains adsorbed to the solid support, wherein the buffer composition in step (a) is of different composition from the solution in step (b); and (c) after step (b), eluting the target molecule from the neutralized solid support by contacting the solid support with a solution of different composition from the solution in step (b), thereby purifying the target molecule in a sample.