| 摘要 |
The present invention relates to a kit for amplifying and detecting a Chikungunya virus (CHIKV) nucleic acid sequence, comprising: (a) a first primer up to 100 bases long and comprising a target-complementary 3' terminal sequence consisting of 15-43 contiguous bases of SEQ ID NO:35, which is in the gene encoding the E1 protein, (b) a second primer up to 100 bases long and comprising a target-complementary 3' terminal sequence consisting of 15-41 contiguous bases of SEQ ID NO:56,which is in the polyprotein gene region, the target-complementary 3' terminal sequence of the first and second primers being fully contained within the sequences of SEQ ID NO:35 and SEQ ID NO:56, respectively, and the first and second primers optionally comprising a second primer 5' sequence that is not complementary to CHIKV nucleic acids; and (c) a hybridization probe for detecting a nucleic acid amplification product synthesized using the primers, wherein the primers and the hybridization probe are in packaged combination with each other. |
