| 摘要 |
FIELD: medicine.SUBSTANCE: allergen tuberculo-protein is produced by culturing tuberculosis mycobacteria for 6-8 weeks on a synthetic nutrient medium. On completion of the culturing procedure, the tuberculous culture is sterilised in a pressurised steam chamber at 100-102°C, 0.05-0.15 kgf/cmfor 55-65 min and filtered on membrane filters having a pore size of 0.45 mcm. The produced filtrates are purified by ultrafiltration with the use of a polysulphone membrane at a graded molecular mass cut-off of 10 kD. The active tuberculo-protein is precipitated with the used of 50% trichloracetic acid. The produced material is purified with the use of ethanol and ether for anaesthesia in a centrifuge by centrifugation at 1,500-2,500rpm at 0 to 5°C.EFFECT: group of inventions enables producing the diagnostic preparation possessing no sensitizing properties, however having stable quality attributes for a declared shelf life; it provides increasing the tuberculo-protein yield, improving the preparation efficacy, and reducing the amount of ether for anaesthesia.2 cl, 1 tbl, 1 ex |
