Immunochromatographic strip disc for multiplexed detection and detection method using the same

摘要

Provided is a strip-assembled immunochromatographic disc, containing: a base, a lid engaged with the base and a draining piece disposed between the strips on the base and the lid, wherein a sampling opening is disposed on the lid directly facing to the draining piece, and the said sampling opening intercommunicates to a draining groove provided on the inner side of the lid which is formed by a plurality of draining channels; several strip stages are provided on the base with their location and number corresponding to those of the draining channels provided on the lid, and the edge of the draining piece laps to the sample pads of the strips carried on the stage adjacent to one end of the sampling opening. Also provided is a method of performing multiplexed immnochromatographic detection using the strip disc to accomplish the detection of multiple target analytes in one sample in an assay.

主权项

1. An immunochromatographic strip disc for multiplexed detection, said strip disc being one of a circle, a square, a rectangle, a diamond, or a regular polygon, comprising:
a base; a lid engaged with the base, wherein an inner side of such lid comprises a plurality of draining channels disposed thereon; a plurality of immunochromatic test strips disposed on the base, each test strip comprising a sample pad, an analytical membrane and a sticky substrate; a plurality of strip stages, disposed on the base, on which said test strips are carried, wherein said strip stages are arranged in a central or axial symmetry, the location and number of such strip stages corresponding to the location, number and position of each draining channel of the plurality of draining channels disposed on the lid, each test strip being fixed to the base via its sticky substrate; a sampling opening in the lid directly facing a draining piece and positioned at the geometric center of the lid, wherein said sampling opening intercommunicates to a draining groove provided on the inner side of the lid, said draining groove formed by the plurality of draining channels, wherein an edge of the draining piece laps to the sample pads adjacent to one end of the sampling opening; and wherein each draining channel comprises two upper fringes and a lower fringe, inner sides of the two upper fringes occlude with an edge of each strip stage and extend directly to the underside of the base, and wherein the lower fringe chucks tightly to the sample pad of the test strip, and wherein all draining channels are connected to one another sequentially.