发明名称 |
IMPROVED METHODS OF CELL CULTURE FOR ADOPTIVE CELL THERAPY |
摘要 |
An improved method of culturing cells for cell therapy applications that includes growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cm2 if the growth surface is not comprised of gas permeable material and up to 2 ml/cm2 if the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5x106 cells/cm2 at the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25 x105 cells/cm2. |
申请公布号 |
SG11201407226W(A) |
申请公布日期 |
2014.12.30 |
申请号 |
SG11201407226W |
申请日期 |
2013.05.20 |
申请人 |
WILSON WOLF MANUFACTURING CORPORATION |
发明人 |
VERA, JUAN F.;ROONEY, CLIONA M.;LEEN, ANN M.;WILSON, JOHN R. |
分类号 |
C12M1/04;C12M1/12;C12N5/0783 |
主分类号 |
C12M1/04 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|