发明名称 |
REAL-TIME MULTIPLEXED HYDROLYSIS PROBE ASSAY USING SPECTRALLY IDENTIFIABLE MICROSPHERES |
摘要 |
Methods and compositions for the detection and quantification of nucleic acids are provided. In one embodiment, a sample is contacted with a primer and a quencher-probe complementary to a target nucleic acid. The quencher-probe is complementary to an anti- probe that comprises a reporter and is attached to a solid support. Thus, hybridized probe is cleaved with a nucleic acid polymerase having exonuclease activity to release the quencher from the probe. The presence of the target nucleic acid is then detected and/or optionally quantified by detecting an increase in signal from the fluorescent reporter on the solid support. |
申请公布号 |
WO2014205083(A1) |
申请公布日期 |
2014.12.24 |
申请号 |
WO2014US42950 |
申请日期 |
2014.06.18 |
申请人 |
LUMINEX CORPORATION |
发明人 |
WHITMAN, DOUG;SCHRADER, BRIAN |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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