| 摘要 |
A device is described that overlays a first fluid, such as blood or a cellular suspension onto a base material, such as a density gradient. In some embodiments, the fluid layering device includes a cylindrical reservoir, a fluid barrier, a coupling extension, a plunger, and an exhaust vent. The fluid layering device can be coupled through its coupling extension to an open end of a container, such as a conical centrifuge tube, including the density gradient. Once attached, the plunger may be lowered to a position above the surface of the density gradient. A first fluid may flow from the reservoir into the conical tube across the plunger, so that a suitable overlay is formed without substantially disturbing a surface of the density gradient. The plunger may be buoyed upward by the first fluid during operation, providing a constant regulation of flow throughout overlaying, regardless of the care and skill of the user. |
| 主权项 |
1. A fluid layering device configured to control a flow of fluid into an open-ended container, comprising:
a fluid reservoir, comprising
a housing defining an open-ended reservoir,a coupling portion attached to the housing, and configured for inserting into an open-ended container for sealing engagement along an interior surface of the open-ended container, anda central opening in the open-ended reservoir; and a plunger, comprising
a shaft inserted through the central opening in the open-ended reservoir, anda cylindrical cup attached to a lower end of the shaft. |
