发明名称 |
CELL SHEET HAVING HYALURONIC ACID PRODUCTION ABILITY AND METHOD FOR PREPARING THE SAME |
摘要 |
A cell sheet having a high hyaluronic acid production ability can be typically prepared by a method in which, on a cell culture support having a surface coated with a polymer which changes a hydration ability in a temperature range of 0-80°C, cells with increased stem cell content are cultured in a temperature range where the polymer exhibits a weak hydration ability, and then the temperature of a culture solution is changed to a temperature at which the polymer exhibits a strong hydration ability, so that the cultured cells are detached in the form of sheet. |
申请公布号 |
EP2682458(A4) |
申请公布日期 |
2014.12.03 |
申请号 |
EP20120752625 |
申请日期 |
2012.02.28 |
申请人 |
CELLSEED INC.;NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY |
发明人 |
SUGIYAMA, HIROAKI;SAKAI, HIDEAKI;SHINOHARA, YASURO;TAKEGAWA, YASUHIRO |
分类号 |
C12N5/071;A61K35/00;A61K35/12;A61L27/00;A61L27/16;A61L27/38;A61P9/00;A61P17/00;A61P19/02;A61P27/02;C12N5/079 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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