发明名称 |
High density self-contained biological analysis |
摘要 |
Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include high density nucleic acid amplification and detection and immuno-PCR. |
申请公布号 |
US8895295(B2) |
申请公布日期 |
2014.11.25 |
申请号 |
US200712515123 |
申请日期 |
2007.11.14 |
申请人 |
Biofire Diagnostics, LLC |
发明人 |
Ririe Kirk M.;Kessler Gary Clark;Jones David E.;Poritz Mark Aaron |
分类号 |
C12M1/34;G01N33/543;B01L3/00;C12Q1/68;C12P19/34;C07H21/02;G01N35/00 |
主分类号 |
C12M1/34 |
代理机构 |
Myers Bigel Sibley & Sajovec, PA |
代理人 |
Myers Bigel Sibley & Sajovec, PA |
主权项 |
1. A container for performing a plurality of reactions on a fluid sample in a closed system comprising
a high density reaction zone provided in the closed system comprising
a channel for receiving the sample into the high density reaction zone,a plurality of high density reaction wells fluidly connected to the channel, each high density reaction well comprising one or more reagents, anda barrier layer disposed between the channel nd the high density reaction wells that minimizes cross-contamination between high density reaction wells upon introduction of the sample to the high density reaction zone, wherein the barrier layer is a pierced layer having one or more piercings per reaction well, and wherein the piercings extend through the pierced layer and are large enough to allow the fluid sample to pass in the presence of a force, but small enough to substantially prevent the fluid sample from passing absent the force, wherein the high density reaction zone is configured such that the sample received through the channel flows across the pierced layer, over all of the piercings, and through the piercings into the high density reaction wells. |
地址 |
Salt Lake City UT US |