OPTICAL PROJECTION TOMOGRAPHY MICROSCOPY (OPTM) FOR LARGE SPECIMEN SIZES

摘要

Claimed is an imaging and diagnostic system and method for focal scanning of a specimen using optical projection tomographic microscopy and computer generation of three-dimensional images. One embodiment comprises a light source and an imaging system having an adjustable focal position which acquires a plurality of digital 2D projection images of biological tissue placed within a specimen tube that translates and rotates past an optical lens in a helical pattern. A computer captures the images and generates a 3D composite image. Also claimed is a system and method for preparing a specimen for optical microscopy. One embodiment comprises fixing, staining, and/or optically clearing biological tissue within a microfluidic specimen chamber prior to placement in a specimen tube.

主权项

1. A method to prepare a specimen of biological tissue for 3D focal scanning optical projection tomography, comprising:
introducing the specimen of biological tissue into a specimen channel of a microfluidics device; moving the specimen of biological tissue from the specimen channel into a specimen chamber of the microfluidics device; and preparing the specimen of biological tissue within the specimen chamber for optical imaging.