Methods of amplifying a target sequence of a 16S rRNA or 16S rDNA in a prokaryotic species

摘要

Disclosed herein is a method of amplifying a target nucleotide sequence in 16S rRNA or in 16S rDNA that includes (a) contacting a sample that includes a 16S rDNA and/or the reverse transcription product of a 16S rRNA with an oligonucleotide primer having the nucleotide sequence of TCC TAC GGG AGG CAG CAG (SEQ ID NO 1) and an oligonucleotide primer comprising the nucleotide sequence of CGG TTA CCT TGT TAC GAC TT (SEQ ID NO 2); and (b) performing a primer-dependent nucleic acid amplification reaction to amplify the target nucleotide sequence in the 16S rRNA or the 16S rDNA. Also included are methods of measuring the prokaryotic content of a sample and/or determining the taxonomic classification of a prokaryotic organism in a sample by detecting and/or analyzing the amplification product.

主权项

1. A method of amplifying a target nucleotide sequence in 16S rRNA or in 16S rDNA comprising
(a) contacting a sample comprising a 16S rDNA and/or the reverse transcription product of a 16S rRNA with an oligonucleotide primer consisting essentially of the nucleotide sequence of SEQ ID NO 1 and an oligonucleotide primer consisting essentially of the nucleotide sequence of SEQ ID NO 2; and (b) performing a primer-dependent nucleic acid amplification reaction to amplify the target nucleotide sequence in the 16S rRNA or the 16S rDNA.