发明名称 |
Cytochrome <i>C </i>synthesis inhibitors |
摘要 |
The invention provides methods for identifying a compound that inhibits cytochrome c synthesis. This invention further provides a method for the high throughput screening of compounds that inhibit cytochrome c synthesis. |
申请公布号 |
US8889378(B2) |
申请公布日期 |
2014.11.18 |
申请号 |
US201012831643 |
申请日期 |
2010.07.07 |
申请人 |
Washington University in St. Louis |
发明人 |
Kranz Robert |
分类号 |
C12P21/04;C12Q1/02;G01N33/573 |
主分类号 |
C12P21/04 |
代理机构 |
Polsinelli PC |
代理人 |
Polsinelli PC |
主权项 |
1. A method for identifying a compound that inhibits cytochrome c synthesis in a bacterial cell, the method comprising:
a. contacting a transfected bacterial cell with a compound, the bacterial cell being transfected with a first expression vector encoding one or more Ccs protein selected from the group consisting of (i) CcsA and CcsB, and (ii) a fusion of CcsA and CcsB, and a second expression vector encoding a cytochrome c reporter protein, wherein the bacterial cell comprises a chromosomal mutation such that expression of a nucleic acid sequence encoding one or more Ccm protein is reduced and the bacterial cell cannot synthesize cytochrome c; and b. determining the amount of cytochrome c reporter protein produced in the presence of the compound relative to the amount produced in the absence of the compound, wherein a decrease in amount is an indication that the compound inhibits the synthesis of cytochrome c. |
地址 |
St. Louis MO US |