MESOTHELIN-SPECIFIC IMMUNOCYTOKINE AND USE THEREOF

摘要

Described herein is the development of an immunocytokine based on interleukin-12 (IL12) and the mesothelin-specific SS1 Fv. The IL12-SS1 (Fv) immunocytokine was produced in insect cells using a baculovirus expression system. The SS1 single-chain Fv was fused to the C terminus of the p35 subunit of IL12 through a short linker. The single-chain IL12-SS1 (Fv) immunocytokine binds native mesothelin proteins on mesothelioma and ovarian cancer cells, as well as recombinant mesothelin. The recombinant immunocytokine retains sufficient bioactivity of IL12 and significantly inhibits human malignant mesothelioma in an animal model.