SELF-CELL PENETRATING FLUORESCENT PEPTIDE BIOSENSORS TO PROBE AND QUANTIFY CDK/CYCLIN KINASES

摘要

The present invention relates to compounds comprising a polypeptide and a fluorophore, said said compounds being able to penetrate directly into living cells and to bind to CDK/Cyclin complexes. Compounds according to the invention are therefore able to probe the relative abundance of these protein complexes. The present invention also relates to a method for determining the presence of CDK/Cyclin complexes in cell samples through a ratiometric quantitation strategy using compounds according to the invention. The invention finally relates to the use of said compounds and/or of compositions comprising said compounds for in vitro detecting the presence and/or the level of expression of CDK/Cyclin complexes, for high throughput and high content screening of libraries of compounds and for medical imaging.