SUGARCANE BACILLIFORM VIRAL (SCBV) ENHANCER AND ITS USE IN PLANT FUNCTIONAL GENOMICS

摘要

Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4× array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.

主权项

1. A chimeric transcription regulatory region comprising:
one or more copies of the sugarcane bacilliform viral (SCBV) enhancer element shown in position 337 to position 618 of SEQ ID NO: 1, or a homolog thereof; and operably linked thereto, a promoter comprising an RNA polymerase binding site and a mRNA initiation site, wherein when a nucleotide sequence of interest is transcribed under regulatory control of the chimeric transcription regulatory region, the amount of transcription product is enhanced compared to the amount of transcription product obtained with the chimeric transcription regulatory region comprising the promoter and not comprising the SCBV enhancer sequence(s).