Method for Extracting and Analysing of Intracellular Metabholic Profile in Microorganisms

摘要

PURPOSE: An extraction and analysis method of microorganisms for acquiring an intracellular metabolic profile is provided to obtain a metabolic profile of high productivity and high yield and to construct an optimal method for preparing intracellular metabolites. CONSTITUTION: A method for acquiring an intracellular metabolic profile in microorganisms comprises the steps of: suspending microorganism biomass in cold methanol and freezing the biomass; thawing the biomass and repeating the steps; collecting a suspension of the metabolites released from the biomass; and performing mass spectrometry of the suspension and identifying the intracellular metabolites.