RECOMBINANT INFLUENZA VIRUS HIGHLY EXPRESSING HA PROTEIN AND PREPARATION METHOD AND USE THEREOF

摘要

A PR8 recombinant influenza virus contains an HA and/or NA gene of H1, H3, H4, H5, H6, H7, H9 or H10 subtype influenza virus, and 6 internal genes (PB1, PB2, PA, NP, M and NS genes) of PR8 virus, in which the NS and/or NP gene have the following mutation sites: the NS2 protein encoded by the NS gene has an E67S point mutation, E74S point mutation, or E67S/E74S point mutation, and the NP protein encoded by the NP gene has a G132A point mutation.

主权项

1. A PR8 recombinant influenza virus comprising
an HA and/or NA gene of an influenza virus, and 6 internal genes (PB1, PB2, PA, NP, M and NS genes) of PR8 virus,
wherein the NS and/or NP gene comprises the following mutation sites:
the NS2 protein encoded by the NS gene has an E67S point mutation, E74S point mutation or E67S/E74S point mutation, andthe NP protein encoded by the NP gene has a G132A point mutation, andwherein the influenza virus is one selected from the group consisting of an H1 subtype influenza virus other than PR8 virus, an H3 subtype influenza virus, an H4 subtype influenza virus, an H5 subtype influenza virus, an 116 subtype influenza virus, an H7 subtype influenza virus, an H9 subtype influenza virus, and an H10 subtype influenza virus.