| 摘要 |
<p>Disclosed is a process for the production of human growth hormone (hGH) comprising:, (i) fermenting human growth hormone producing E. coli cells;, (ii) recovering inclusion bodies from the E. coli cells and dissolving the recovered inclusion bodies at an alkaline pH to provide dissolved hGH;, (iii) optionally lyophilizing dissolved hGH;, characterized in that the fermentation is carried out in a culture medium comprising manganese, zinc, cobalt, molybdenum, calcium, copper, and boron as trace elements., Further disclosed is a method for reducing the amount of human growth hormone polymeric forms formed during a process for the production of human growth hormone (hGH) comprising the process as defined above.</p> |
