Mutant G-CSF fusion protein, and preparation and use thereof

摘要

The present invention relates to a mutant G-CSF fusion protein. The mutant G-CSF fusion protein is a fusion protein having the activity of stimulating the proliferation of neutrophilic granulocytes, and having a basic structure of G-CSF/carrier protein or carrier protein/G-CSF; wherein the G-CSF moiety comprises multipoint substitutions thus resulting in changes in biological activity and binding affinity. Compared with existing products, the mutant G-CSF fusion protein in the present invention has longer half-life and higher biological activity. Administration of the pharmaceutical preparation containing this mutant G-CSF fusion protein could be used in the treating neutropenia.

主权项

1. A mutant G-CSF fusion protein, comprising a G-CSF moiety and a carrier protein; said G-CSF moiety being derived from a human G-CSF protein with its amino acid sequence set forth in SEP ID NO: 1; said G-CSF moiety comprising at least one of the mutations of K34H, L35I, K40H and L41I; and said mutant G-CSF fusion protein stimulating proliferations of neutrophilic granulocytes.