Bromate ion measurement method

摘要

A method for measuring bromate ion is provided that provides high-sensitivity measurement results more simply and more quickly than conventional bromate ion measurement methods. A fluorescent substance that is quenched by coexistence with bromate ions is added to a sample 130 and the fluorescence intensity of the fluorescent substance after quenching is measured, the measured fluorescence intensity being subtracted from the fluorescence intensity of a standard sample containing no bromate ions to calculated the fluorescence intensity difference. The bromate ion concentration is calculated from the calculated fluorescence intensity difference, using a pre-determined calibration line between the fluorescence intensity difference and the bromate ion concentration.

主权项

1. A method for measuring bromate ions in a sample, comprising the steps of:
mixing trifluoperazine with the sample, wherein fluorescence emission from trifluoperazine is quenched in the presence of bromate ions under acidic conditions; measuring florescence intensity emitted from the mixture of trifluoperazine and the sample under acidic conditions; subtracting the measured fluorescence intensity emitted from the mixture of trifluoperazine and the sample from a measured fluorescence intensity emitted from a standard sample that does not contain bromate ions to calculate a fluorescence intensity difference; and determining the bromate ion concentration in the sample from the calculated fluorescence intensity difference using a pre-determined calibration line that relates fluorescence intensity difference with bromate ion concentration, wherein the method further comprises a step of adding a chelating agent to the sample before the step of mixing trifluoperazine and the sample, and wherein the chelating agent is ethylene diamine tetraacetate.