Array for detecting microbes

摘要

The present embodiments relate to an array system for detecting and identifying biomolecules and organisms. More specifically, the present embodiments relate to an array system comprising a microarray configured to simultaneously detect a plurality of organisms in a sample at a high confidence level.

主权项

1. A method comprising:
applying a sample comprising nucleic acids derived from a plurality of microorganisms representing a plurality of operational taxonomic units (OTUs) to a system, the system comprising (i) a first probe set comprising a plurality of different first nucleic acid probes, each of which is complementary to a 16S rRNA or rDNA sequence that is present only within a first OTU, (ii) a second probe set comprising a plurality of different second nucleic acid probes, each of which is complementary to a 16S rRNA or rDNA sequence that is present in more than one OTU but collectively are present only in a second OTU, wherein the second OTU is different than the first OTU, and (iii) a third probe set consisting of a plurality of different third nucleic acid probes for detecting a third OTU, wherein the third nucleic acid probes are complementary to 16S rRNA or rDNA sequences collectively present in the third OTU and another OTU, and are selected to minimize the number of putative cross-reactive OTUs, wherein the third OTU is different from the second OTU and the first OTU; and detecting hybridization between the nucleic acids derived from the plurality of microorganisms and the first, second and third probe sets, and identifying the presence and/or the quantity of microorganisms from the first, second and third OTUs based on the hybridization.