发明名称 |
METHODS, PEPTIDES AND BIOSENSORS USEFUL FOR DETECTING A BROAD SPECTRUM OF BACTERIA |
摘要 |
Described herein are methods of detecting a wound infection and for detecting the presence or absence of bacteria, for example, wound bacteria in a sample, by contacting a sample with a peptide substrate derived from the modification of the reactive site loop (RSL) domain of the .alpha. l- proteinase inhibitor. In the current invention, we have demonstrated that these peptide substrates without the alpha 1 protein can be efficiently used as peptide substrates. The modification or the absence of modification of this peptide substrate by the enzyme produced and/or secreted by the bacteria, can serve as an indicator for the presence or absence of the bacteria in the sample. The present invention also features a biosensor for detecting the presence or absence of bacteria in a sample. |
申请公布号 |
CA2557612(C) |
申请公布日期 |
2014.06.17 |
申请号 |
CA20042557612 |
申请日期 |
2004.11.03 |
申请人 |
ETHICON, INC.;EXPRESSIVE CONSTRUCTS, INC. |
发明人 |
SEBASTIAN, SHITE;COLPAS, GERARD J.;ELLIS-BUSBY, DIANE L.;HAVARD, JENNIFER M.;SANDERS, MITCHELL C. |
分类号 |
G01N33/569;C12Q1/04;C12Q1/37;G01N33/543 |
主分类号 |
G01N33/569 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|