IDENTIFICATION OF A WEST NILE VIRUS CD4 T CELL EPITOPE AND USE THEREOF

摘要

Described herein is the identification and of a potent West Nile virus (WNV) CD4 positive T cell epitope and its use for increasing the immunogenicity of heterologous flavivirus vaccines, such as dengue virus type 2 (DENV-2) DNA and virus-like particle (VLP) vaccines. Also described are methods for the identification of potent T cell epitopes to enhance immunogenicity of multivalent vaccines.

主权项

1. An isolated mutant flavivirus E-glycoprotein polypeptide, wherein the polypeptide comprises an isoleucine at position 474, a threonine at position 484, a valine at position 488 and a leucine at position 493, each numbered with reference to the West Nile virus E-glycoprotein polypeptide sequence of SEQ ID NO: 38, wherein the flavivirus is not West Nile virus.