Methods For Making Multimeric Polypeptides

摘要

Detailed herein are contiguous, multimeric, multispecific polypeptides, nucleic acids encoding such polypeptides, and methods for making such polypeptides and nucleic acids.

主权项

1. A nucleic acid molecule encoding a contiguous, multimeric polypeptide comprising at least two subunits, each of which includes at least a functional domain (FD) and an interaction domain (ID), wherein the nucleic acid molecule comprises:
i) a nucleic acid portion comprising a nucleotide sequence that encodes a functional domain (FD1) that binds to a first binding site; ii) a nucleic acid portion comprising a nucleotide sequence that encodes an interaction domain (ID1); iii) a nucleic acid portion comprising a nucleotide sequence that encodes a functional domain (FD2) that binds to a second binding site; and iv) a nucleic acid portion comprising a nucleotide sequence that encodes an interaction domain (ID2); wherein the nucleic acid molecule further comprises at least one nucleic acid portion comprising a nucleotide sequence encoding a polypeptide linker, which polypeptide linker includes at least one protease cleavage site; wherein ID1 and ID2 are capable of associating with each other, and wherein the contiguous, multimeric polypeptide is multispecific.