Surface plasmon resonance sensor

摘要

An SPR sensor comprising a thin conducting layer comprising at least one conductive element formed on a surface of a transparent substrate, a light source that illuminates an interface between the conducting layer and the substrate, a photosensitive surface that generates signals from light reflected from the interface, a flow cell formed with at least one flow channel having a lumen defined by a wall formed from an elastic material and from a region of the conducting layer, and at least one hollow fluid-providing flow control apparatus having a lumen and an orifice communicating with its lumen. Fluid flow is enabled between the flow channel and the lumen of the flow control apparatus by forcing an end of the flow control apparatus through the elastic material so that the orifice communicates with the flow channel lumen.

主权项

1. A surface plasmon resonance (SPR) system using a ligand, the system comprising:
a) a thin conducting layer suitable for SPR formed on a transparent substrate, the conducting layer comprising a plurality of electrodes; b) at least one flow channel crossing the electrodes at crossover regions; c) a power supply connected to the electrodes, and differently electrifying the electrodes at two different cross-over regions, such that the ligand flowing through the flow channel is attracted to and immobilized on the electrode at one of the cross-over regions, and repelled by and substantially prevented from immobilizing on the electrode at the other of the cross-over regions; and d) a photosensitive surface that generates SPR signals in response to light reflected from the crossover region at which the ligand is immobilized, that can be used to determine an SPR parameter, and generates reference SPR signals in response to light reflected from the crossover region at which the ligand is prevented from immobilizing, that can be used to normalize and correct the SPR signals from the crossover region at which the ligand is immobilized.