| 摘要 |
The present invention allows a TET1 protein to be more stably expressed in human differentiated pluripotent stem cells than in the past by, inter alia, substituting the second amino acid from the amino terminal of a TET1 protein with a different amino acid. Furthermore, it is possible to quickly eliminate the expression of, inter alia, NANOG, which is an inhibitor of differentiation induction, and promote the expression of factors related to differentiation induction by introducing a variant TET1 protein to a differentiated pluripotent stem cell. The present invention provides a method for manufacturing differentiated pluripotent stem cells with increased differentiation performance, and a substance that is useful to said method. |
