发明名称 |
METHOD FOR THE GENERATION OF A MONOCLONAL PLANT CELL LINE |
摘要 |
The invention provides a method for the generation of a monoclonal plant cell line from a heterologous population of plant cells, comprising the following steps: (a) provision of a heterologous population of plant cells; (b) preparation of protoplasts from said heterologous population of plant cells; (c) separation of single protoplasts by subjecting the preparation of protoplasts to flow cytometric sorting; (d) regeneration of a separated single transformed protoplast until the formation of a microcolony by co-cultivation in the presence of feeder cell material; (e) removal of the microcolony from the feeder cell material and cultivation of the microcolony until the formation of a monoclonal plant cell line. |
申请公布号 |
KR20140048880(A) |
申请公布日期 |
2014.04.24 |
申请号 |
KR20137032519 |
申请日期 |
2011.06.07 |
申请人 |
FRAUNHOFER-GESELLSCHAFT ZUR FORDERUNG DER ANGEWANDTEN FORSCHUNG E. V. |
发明人 |
KIRCHHOFF JANINA;SCHILLBERG STEFAN;SCHIERMEYER ANDREAS;SCHINKEL HELGA;FISCHER RAINER |
分类号 |
A01H4/00;A01H1/06;C12N15/82 |
主分类号 |
A01H4/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|