| 摘要 |
<p>The invention relates to a method of detecting a presence and/or measuring a quantity of an analyte in a sample by a nucleic acid amplification reaction, said method comprising: acquiring a fluorescence intensity of the analyte for each amplification reaction cycle of the nucleic acid amplification reaction (810) and creating from said fluorescence intensity a growth signal being indicative of a fluorescence intensity over the cycles, determining an intercept value of the signal (820), determining a baseline of the signal (830), determining a maximum growth value of the signal (840), normalizing the signal by modifying said signal (850), wherein the modification comprises calculating a difference of the signal and the baseline and dividing the difference by a divisor, wherein the divisor is proportional to a combination of the intercept and the maximum growth value, wherein the normalized signal is dimensionless, and processing (860) the normalized signal in order to determine a cycle number at which the normalized signal exceeds the baseline (870) for detecting the presence and/or measuring the quantity of the analyte (880).</p> |
