| 摘要 |
PURPOSE: A malignant undifferentiated cancer stem cell target peptide is provided to effectively and accurately deliver anti-cancer drug to tumor cells by targeting nestin-positive cells of tumor cells. CONSTITUTION: A malignant undifferentiated cancer stem cell target peptide GICT has AQYLNPS sequence which can selectively recognizes and combines with the malignant undifferentiated tumor stem cells. A marker for diagnosing malignant cancers uses the cancer stem cell target peptide GICT having AQYLNPS sequence. A method for screening the cancer stem cell target peptide, which can selectively recognize and combine with the malignant undifferentiated cancer stem cells, uses phage display technique. The method for sorting and inspecting the cancer stem cell target peptide comprises the following steps: prying the cancer stem cell target peptides through sorting the cancer stem cell bonded phage and analyzing the individual phage combination aspect by using the page display technique; analyzing combining ability of the pried cancer stem cell target peptide with the various cells; sorting and verifying the protein bonded with the pried cancer stem cell target peptide; verifying the cancer tissue transmission ability of the cancer stem cell target peptide in a cancer model mouse; verifying undifferentiated cancer stem cell specific targeting ability of the cancer stem cell target peptide in the brain tumor organization; and confirming the cancer cell growth inhibition ability in the tumor model mouse of the tumor stem cell target peptide. |
