A vector for cell surface expression of bovine IgG Fc domian, a host cell transformed with the vector and a manufacturing method of vaccine against viruses related to cattle diseases using the host cell

摘要

PURPOSE: A vector for expressing IgG Fc domain on cell surface is provided to easily produce a vaccine having excellent immunogenicity to virus relating to bovine diseases. CONSTITUTION: A vector for expressing bovine IgG Fc domain contains a gene encoding the IgG Fc domain and gene encoding transmembrane domain of bovine transferring receptor. The gene encoding the transmembrane domain of bovine transferring receptor comprises a sequence of sequence number 5. The gene encoding Fc domain is a gene encoding hinge, CH2 and CH3. A method for manufacturing the vaccine to bovine disease-relating virus comprises: a step of infecting bovine disease-relating virus to a host cell using the expression vector to proliferate the virus; and a step of manufacturing the live vaccine or inactivated vaccine. The virus is bovine viral diarrhea virus (BVDV), ephemeral fever virus (BEF), bovine corona virus (BCV), bovine rotavirus (BoRotaV), bovine rhi notracheitis virus (IBRV), foot-and-mouth disease virus (FMDV), bovine parainfluenza virus (PI-3), blue tongue virus (BTV), bovine leukemia virus (BLV), cattle respirator syncytia virus (BRSV), akabane virus (AKV) or Ibaraki virus (IBV).