发明名称 |
PROTEIN PRODUCTION METHOD |
摘要 |
<p>The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.</p> |
申请公布号 |
KR20140012035(A) |
申请公布日期 |
2014.01.29 |
申请号 |
KR20137015380 |
申请日期 |
2011.12.14 |
申请人 |
RESEARCH ORGANIZATION OF INFORMATION AND SYSTEMS;KYOWA HAKKO KIRIN CO., LTD. |
发明人 |
KAWAKAMI KOICHI;KUROKAWA MEGUMI;YAMAGUCHI KEINA;OGAWA RISA;TSUKAHARA MASAYOSHI;HAYASHI YOKO |
分类号 |
C12N15/09;C12N5/10;C12P21/02;C12P21/08 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|