| 摘要 |
The invention relates to methods for expressing nucleic acid sequences in prokaryotic host cells. According to said methods, at least one DNA construct that can be episomally replicated in said host cells and comprises a nucleic acid sequence that is to be expressed is introduced into the host cells under the transcriptional control of an L-rhamnose-inducible promoter, said promoter being heterologous relative to said nucleic acid sequence, and expression of the nucleic acid sequence is induced by adding R-rhamnose. The inventive methods are characterized by the fact that the prokaryotic host cell used is at least deficient in an L-rhamnose isomerase. |
