Method for Detecting a Target Analyte Using a Self-Cleaving Synthetic Molecule

摘要

One aspect of the present disclosure relates to a method for determining the amount of a target analyte in a sample. One step of the method can include contacting the sample with a self-cleaving synthetic molecule to form a first treated sample. The self-cleaving synthetic molecule can include at least one target analyte-specific aptamer. Next, the first treated sample can be contacted with at least one actuating molecule to form a second treated sample. The second treated sample is then subjected to a target amplification assay. A detectable signal is produced by the target amplification assay when the target analyte is present in the sample.