| 摘要 |
It is an object of the present invention to improve the performance of gradient liquid transfer in nano/micro liquid chromatographs when solvents are mixed. In a gradient liquid transfer device and method for a nano/micro liquid chromatograph, an opening and closing unit ( 160 ) capable of opening and closing a solvent passage ( 150 ) for transferring a secondary solvent is provided in the vicinity of a solvent mixing section ( 152 ) in a liquid transfer system ( 130 ); a passage from a metering pump ( 146 ) of a liquid transfer section ( 142 ) to the opening and closing unit ( 160 ) is filled with the secondary solvent in advance; and an appropriate pressure is applied to the secondary solvent. Therefore, the entry of a primary solvent into the solvent passage for transferring the secondary solvent is suppressed. In another gradient liquid transfer device and method for a nano/micro liquid chromatograph, a liquid having a low compression rate is filled, in advance, in a part of a solvent passage subsequent to a metering pump of a liquid transfer section, and only the required amount of a secondary solvent is filled subsequent to the liquid. Therefore, the entry of the primary solvent into a solvent passage for transferring the secondary solvent is suppressed, and liquid-transfer performance is improved.
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