发明名称 |
Fermentation process |
摘要 |
<p>The present invention provides a process for periplasmic expression of a bacterial toxoid comprising the steps of: a) growing a culture of a gram negative host cell in a fermentation medium, wherein the host cell is transformed with a polynucleotide, and wherein the polynucleotide encodes the bacterial toxoid and a periplasmic signal sequence; or providing a gram negative host cell wherein the host cell is transformed with a polynucleotide, the polynucleotide encodes the bacterial toxoid and a periplasmic signal sequence and wherein the gram negative host cell comprises the bacterial toxoid expressed in the periplasm; a(i)) inducing expression of the bacterial toxoid; b) maturing the host cell, wherein the maturing step comprises: I) subjecting the host cell to a pH shock; II) incubating the host cell with no feed addition; and/or III) subjecting the host cell to a temperature below -20°C; and c) extracting the bacterial toxoid from the host cell wherein the extraction process comprises osmotic shock.</p> |
申请公布号 |
AU2012241853(A1) |
申请公布日期 |
2013.11.28 |
申请号 |
AU20120241853 |
申请日期 |
2012.04.12 |
申请人 |
GLAXOSMITHKLINE BIOLOGICALS S.A. |
发明人 |
DEHOTTAY, PHILIPPE MARC HELENE;GOFFIN, PHILIPPE |
分类号 |
C12P21/00;A61K39/02;C07K14/195;C07K14/34 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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