| 摘要 |
PURPOSE: A plant transformation vector, pCMF, for removing a selective marker is provided to enable easy modification of the vector and transduction of a target gene, and to develop marker-free GM(genetically modified) crops. CONSTITUTION: A site-specific recombinase gene, mFLP, has a base in sequence number 1. A vector for transformation in a plant contains the gene, mFLP. The vector contains a stress-induced promoter and recombinase recognition site. The vector is able to remove a selective marker by induction of mFP expression and self-cleavage due to stress. The vector contains MCS(multi-cloning site). A plant is transformed with the vector. Agrobacterium tumefaciens LBA4404 is transformed by the vector and is used as a plant carrier. A transgenic plant is prepared by transforming with the vector and is able to remove the selective marker. |
