发明名称 |
Photoporphyrinogen oxidase having activity of imparting resistance against acifluorfen and gene thereof |
摘要 |
Protoporphyrinogen oxidase having an activity of imparting acifluorfen resistance and gene thereof are provided. Cyanobacterium protoporphyrinogen oxidase gene is identified by introducing a protoporphyrinogen oxidase gene of Arabidopsis into cyanobacterium, disrupting a cyanobacterium gene with a transposon, selecting a mutant strain in which protoporphyrinogen oxidase gene is disrupted, identifying the disrupted protoporphyrinogen oxidase gene, and isolating the disrupted protoporphyrinogen oxidase gene. This procedure is effective as a gene isolation technique when a protein derived from other organism species that is homologous to a known protein (e.g., protoporphyrinogen oxidase from cyanobacterium) can not be found in a gene database of the other species.
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申请公布号 |
US8580940(B2) |
申请公布日期 |
2013.11.12 |
申请号 |
US201213358564 |
申请日期 |
2012.01.26 |
申请人 |
TANAKA AYUMI;TANAKA RYOUICHI;KATO KAZUSHIGE;FUKAGAWA TAKAKO;NIPPON SODA CO., LTD.;NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY |
发明人 |
TANAKA AYUMI;TANAKA RYOUICHI;KATO KAZUSHIGE;FUKAGAWA TAKAKO |
分类号 |
C12N15/09;C12N15/31;C12N15/52;C12N15/65 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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