| 摘要 |
PROBLEM TO BE SOLVED: To provide compositions, kits and related methods for the detection and/or monitoring of Pseudomonas aeruginosa.SOLUTION: The present invention provides compositions, methods and kits for the species-specific detection of Pseudomonas aeruginosa. According to one aspect of the invention, there are provided compositions for use in a Pseudomonas aeruginosa transcription-mediated amplification assay, where the compositions comprise a T7 provider oligonucleotide that targets the complement of a sequence in a region of Pseudomonas aeruginosa 23s rRNA corresponding to bases from about 725-825 of E.coli 23s rRNA reference sequence, and a non-T7 primer oligonucleotide that targets a sequence in a region of Pseudomonas aeruginosa 23s rRNA corresponding to bases from about 845-950 of E.coli 23s rRNA. |
