发明名称 |
Process, vectors and engineered cell lines for enhanced large-scale transfection |
摘要 |
An expression system for stable expression of a gene of interest is described, the expression system comprising: a first vector having an Epstein-Barr virus nuclear antigen-1 (EBNA1) nucleotide sequence encoding a truncated EBNA1 protein, a promoter and a polyadenylation signal for the EBNA1 nucleotide sequence and an oriP nucleotide sequence; and, a second vector having a gene of interest, a promoter and a polyadenylation signal for the gene of interest and an oriP nucleotide sequence. |
申请公布号 |
EP2631297(A1) |
申请公布日期 |
2013.08.28 |
申请号 |
EP20120192258 |
申请日期 |
2009.03.09 |
申请人 |
NATIONAL RESEARCH COUNCIL OF CANADA |
发明人 |
DUROCHER, YVES;LOIGNON, MARTIN |
分类号 |
C12N15/85;C07K14/035;C07K14/05;C07K19/00;C12N15/869 |
主分类号 |
C12N15/85 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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